August 7, 2007
Ran a Touchdown PCR with Pfu and crp* with different amounts of template DNA added:
- 0 (-)
Also ran Taq with 1uL template DNA and a negative. Diluted primers 1:10 before adding. Used protocol from July 25, 2007 PCR run.
Don't understand the gel at all. Negatives came up positive and Pfu positives didn't work. Must have made a mistake so i'm re-running a touchdown PCR with template DNA amounts of:
Also ran a negative and Taq with 1uL template and a Taq negative. Let run overnight