IGEM:PennState/Labbook/NoahJohnson/2007-7-6

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July 6, 2007

  • Yesterdays motility plates found that motility was greatest at 26°C so today we are running 5 motility trials at 26°C with varying concentrations of eiken agar
  • Made motility plates
    • 0.25g tryptone
    • 0.2g NaCl
    • 0.15g sodium citrate
    • 25mL water
    • Eiken Agar:
      • 4 plates of 0.24%
      • 4 plates of 0.26%
      • 4 plates of 0.28%
      • 4 plates of 0.30%
      • 4 plates of 0.32%
  • Ran Gel on Pfx PCR products (Start 2:00)
  • Used Sybr-green
    • Well 1- DNA Ladder
    • Well 2- (-) Pfx
    • Well 3- 56°C Pfx
    • Well 4- 57.1°C Pfx
    • Well 5- 58.6°C Pfx
    • Well 6- 61.3°C Pfx
    • Well 7- 64.9°C Pfx
    • Well 8- 67.5°C Pfx
    • Well 9- 69.1°C Pfx
    • Well 10- 70°C Pfx
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