IGEM:PennState/Labbook/NoahJohnson/2007-7-4

From OpenWetWare

Jump to: navigation, search

July 4, 2007

  • Poured gel
  • Ligation (Started at 12:50):
    • (+) 20uL 1741015-PSB1A2
      • 2uL T4 Ligase buffer
      • 4ul Vector (P1010 in PSB1A2+S+E)
      • 12uL Insert (I741015+S+E)
      • 1uL T4 DNA Ligase
      • 1uL H2O
    • (-) 20uL PSB1A2
      • 2uL T4 Ligase buffer
      • 4uL Vector
      • 1uL T4 DNA Ligase
      • 13uL H2O
  • Ran Transformation
  • Plated on LB Amp

9:30-3:30

Personal tools