IGEM:PennState/Labbook/GalenLynch/2007-7-11

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Contents

July 11th, 2007: 9:30-5:30

Quick Change to strengthen Xyl promoters

XylA to XylF dual promoter/operator

BBa_I741015

GGTGATGGAT GATGTCGTAT TATAGGGCAC TCCCTTTCAG TTTGTCAATT ATGTTATTTC
ACACTGCTAT TGAGATAATT CACAAGTGTG CGCTCGCTCG CAAAATAAAA TGGAATGATG
AAACTGGGTA ATTCCTCGAA GAGAAAAATG CAATAAGTAC AATTGCGCAA CAAAAGTAAG
ATCTCGGTCA TAAATCAAGA AATAAACCAA AAATCGTAAT CGAAAGATAA AAATCTGTAA
TTGACATCCC CTGTTTAGTT GCTATAATTT GGTTACGTTT ATCGCGGTGA TTGTTACTTA
T
Color key: Transcription +1 is in maroon, promoter regions are in green, XylR binding sites are in blue, CRP binding sites are in purple, shared promoter and XylR binding sites are in teal, shared XylR binding sites and CRP binding sites are in indigo, bases modified from wild type using quick change are in orange

Quick Change Primers

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