IGEM:PennState/2007/Labbook/GarrettTobin/2007-8-14

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August 14th

  • Miniprep xylR and B0034
  • Restricted
    • 1.5 hours, double digestion
    • Extracted gel
    • Quatnified DNA
      • 5ng/ul B0034 - 16ng/ul xylR
  • Ligated
    • 9:9 ratio insert to vector
    • 5:10 insert to vector
      • (by volume)
    • 12:30-6:30
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