IGEM:PennState/2007/Labbook/GarrettTobin/2007-7-26

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July 26th Plan for xylR

  • Make 6 different promoter+RBS+xylR constructs with different levels of expression
    • 2 different RBS - B0033 (stregnth .01) - B0034 (streght 10
    • 3 different Promoters - 1, 200, 2000

Today:

  • Transform xylR orf from miniprep
  • Transfrom B0033
    • Both into dh5a
    • B0033 dna from regrestry plates is in my box- labled as exactly that
  • Made sure there was a miniprep for other RBS-B0034\
      • 12:30-5:30

Tomorrow

  • Innoculate
  • Prepare Transformation/Find Minipreps of 3 promoters

Saturday

  • Miniprep, Restrict, Purify, Ligate, Transform
  • Innoculate promoters if needed

Sunday

  • Innoculate
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