IGEM:Peking University/2008/Notebook/Group 2/2008/05/28

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PCR

TITLE: PCR-Budding Yeast Genome-Gal4-K.O.D 50ul
Volume (μL)
Template (Budding Yeast Genome)1
dNTP5
Enzyme1
Buffer5
MgSO4(For KOD)2
F-Gal4-HindIII-ApaI 1
R-Gal4-EcoRI-Xhol1
ddH2O34
conditions
TemperatureTime Length(0:00:00)
94°C02:00
94°C00:2030 cycles
59°C00:3030 cycles
55°C00:3030 cycles
68°C03:0030 cycles
68°C07:00
4°CHold

Failed.



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