IGEM:Peking University/2008/Notebook/Group 1/2008/07/22

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PKU_080722_G1_1


Positive transformation-pGREG504(new)/DH5α/JM109
Picking up clonies



Gel running and testing-hypothesis-promoter's ligation-(lac1/lacS/tet)(SB/SX)AID(BX)
result:no band and snear



Sequencing-ligation product(yesterday)



Ligation test-pGREG504-promoter-old/new ligation buffer

Enzyme cutting and testing-pGREG504-single(sacI/XhoI)-double(sacI&XhoI)



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