PKU_080714_G1_1
- Plasmid Miniprep-pGREG504-pADH-(lac1-1/lac1-2/lac2-1/lac2-2/Control1/Control2/Control3)
- Enzyme cutting-pADH-(lacS/tet)(PCR product)
|
Volume (μL)
|
pADH-(lacS/tet) |
14
|
SacI |
2
|
XhoI |
2
|
M Buffer |
10
|
ddH2O |
72
|
- Enzyme cutting-pGREG504-1
|
Volume (μL)
|
pGREG504-1 |
4
|
SacI |
2
|
XhoI |
2
|
M Buffer |
10
|
ddH2O |
82
|
- lanes:lacS|tet|vector|marker1|marker2
- Enzyme cutting-pGREG504-pADH-(lac1-1/lac1-2/lac2-1/lac2-2/Control1/Control2/Control3)
|
Volume (μL)
|
pGREG504-pADH-(lac1-1/lac1-2/lac2-1/lac2-2/Control1/Control2/Control3) |
5
|
SacI |
1
|
XhoI |
1
|
M Buffer |
2
|
ddH2O |
11
|
|
Volume (μL)
|
pGREG504-pADH-(lac1-1/lac1-2/lac2-1/lac2-2) |
5
|
SphI |
0.2
|
XhoI |
0.2
|
H Buffer |
2
|
ddH2O |
12.6
|
|
Volume (μL)
|
pGREG504-pADH-(Control1/Control2/Control3) |
5
|
BamHI |
0.2
|
XhoI |
0.2
|
K Buffer |
2
|
ddH2O |
12.6
|
|