IGEM:Peking/2007/Switch-Notebook/2007-8-5

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Experimenter: LXL

Precipitate and retract

SDY-EGFP^^(XhoI/SalI)

Ligation

SDY-EGFP    pLX007
SDY-EGFP    pLX003
pLX007      (self-control)
pLX003      (self-control)
SDY-EGFP    (self-control)

Colony PCR test of ligation product

Col.No.  Product
--------------------------
1-46     T1T2-lacZa-pLX003
47,48    lacZa-pLX003(self-ligation-control)
49-76    lacZa-pLX007
78,79    pLX007(self-ligation-control)

8 are incubated.

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