IGEM:Peking/2007/Switch-Notebook/2007-8-4

Experimenter: LXL

Retraction, ligation and transformation

Precipitate and retract

T1T2^^(A/X), pLX007^^(X/S), lacZa-pLX003(A/X)

Ligation and transformation

A+

T1T2^^(A/X)      lacZa-pLX003^^
T1T2^^(A/X)      self-control
lacZa-pLX003^^   self-control
lacZa^^          pLX007^^(X/S)
pLX007^^(X/S)    self-control

Cm+

T1T2^^           pCC010^^
pCC010^^(N/X)    self-control
T1TE^^           pCC010^^(C/S)
T1TE^^           self-control
pCC010^^(C/S)    self-control

Colony PCR test of T1TE-pCC010

Primer: T1TE F/R

48 colonies are tested. 12 are selected for incubation.