IGEM:Peking/2007/Switch-Notebook/2007-8-3

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Experimenter: LXL

Transformation of T1TE-pCC010, T1T2-pCC010

Colony PCR test of CD2, RD2, RS3-pMD18-T

Primers:   MBF/R

6 of each type are correct and sent for sequencing.

Digestion of fragment and plasmid

  2uL    pLX007(4)
  2uL    XhoI
  2uL    SalI
 10uL    10*H buffer
 84uL    H2O
--------------------
100uL    total
  2uL    SD-lacZa-pLX003
  2uL    XhoI
  2uL    AatII
 10uL    T buffer
 10uL    BSA
 84uL    H2O
--------------------
100uL    total
 22uL    T1T2^^(AatII/XhoI)
  2uL    XhoI
  2uL    AatII
 10uL    T buffer
 10uL    BSA
 54uL    H2O
--------------------
100uL    total
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