IGEM:Peking/2007/Switch-Notebook/2007-8-20

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Contents

digestion some plasmids

Image:PKU Switch 07-8-20 R3,SS2.jpg

Image:PKU Switch 07-8-20 R3,SS2 .jpg

colony PCR of RD2, RD3, RS2, CD1

no one is right

ligation of lacI and pcc009

to prepare enough supplies

re-PCR some double promoers

Image:PKU Switch 07-8-20 double promoters 1.jpg

CD1, SS1, RS1, RS2, RD2, RD3(68), RD3(70)

after rescue from the gel, run a electrophoresis to check:

Image:PKU Switch 07-8-20 double promoters 2.jpg

CD1, SS1, RS1, RS2, RD2, RD3

re-digestion of GFP-plx007 3uL

usage: XhoI and XbaI in 100uL solution

transformation of lacI-pcc009

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