IGEM:Peking/2007/Switch-Notebook/2007-8-19

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experimenter: WMC, CDZ

Contents

precipitation of RD3, RD2, RS2, CD1

but the electrophoresis shows no visible signs, which means failure

ligation

with GFP-plx007, all use the proportion 4:1

miniprep of SS3, RS3,all together 30

re-digestion of RD3, RD2, RS3, CD1

template: 20uL XhoI 2uL SpeI 2uL H beffer 10uL ddH2O 66uL for a shortage of template, we just use 10-15uL template in each solution

Sequencing results come back, the results are good!

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