IGEM:Peking/2007/Switch-Notebook/2007-8-11
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colony PCR of T1TE-T1T2-pcc010 and T1T2-pcc010 self-ligation
the results are strange and hard to explain, PCR always tricky???
re-PCR lac, rec, sul, ss1, ss2, ss3, sd1-3
rescue through gel and digest them
prepare some Amp+ LB plate
colony PCR of yesterday's plate
sula-GFP-plx007
lac-GFP-plx007
ss2-GFP-plx007
re-digestion of GFP-plx007
xbaI and XhoI
in the result, two bands through single enzyme digestion! Two sites for both XhoI and XbaI? seem to be impossible! maybe PCR or other procedure included some other DNA here, we need more thorough check about all these things!!!
re-PCR double promoters
positive transformation
to prepare for enough supplies
