IGEM:Peking/2007/Switch-Notebook/2007-7-28
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rescue of digestion production of XhoI/SalI
double digestion test of plx008/009-lacZa
using two systems of ScaI/SalI and XhoI/ScaI
eletrophoresis to check the result
Pick 12 colonies of pcc009-MCS and cultivate
lacZa-T single digestion test by SalI or XhoI
RS1-3, 1-6, 1-8, 2-10 and RD1-1 miniprep by kit and stored
single digestion of RD and RS
plasmid 3uL
SalI 0.5uL
H buffer 2uL
ddH2O 14.5uL
