IGEM:Peking/2007/Switch-Notebook/2007-7-15

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Experimenter: LXL, MHS

Contents

Contents

配酚氯仿

配1*TAE+EB

EB浓度加倍用于看pLX007

Single digestion test of pLX007

  • Enzyme: PstI/ScaI/NdeI
  • Plasmid: 2uL

Image:PKU Switch 07-7-15 PLX007 single digestion test.jpg

l to r: pLX007-1(P/S/N), pLX007-2(P/S/N), pLX007-3(P/S/N), pLX007-4(P/S/N), Kit2(P/S/N), Marker, pLX007-4

Plasmid还看得很清, 但是酶切完全弥散了. pLX007作载体还是相当有问题啊.

今天晚上重新做阳转吧. 用测序的那管质粒好了.

CXY昨天PCR的东东

CD2大小差不多, 不过太稀了. 回收之后2uL看不到.

还是用temperature PCR试一下. 呼唤NM师兄!

Single digestion test of plasmid prepared yesterday (pLX007-lacZa)

  • Enzyme: ScaI & PstI

Image:PKU Switch 07-7-15 PLX007-lacZa plasmid single digestion test.jpg

l to r: pLX007-lacZa^(ScaI)(3,4,33,34), pLX007-lacZa^(Pst2)(1,2,31,32), Marker

可以切开的. ScaI的效率不高.

Double digestion test of plasmid prepared yesterday (pLX007-lacZa)

  • Enzyme: PstI/XbaI
  • Plasmid: 10uL
  • PstI: 0.2uL
  • XbaI: 0.2uL
  • M buffer: 2uL
  • H2O 8uL
  • total: 20uL

Image:PKU Switch 07-7-16 PLX007-lacZa PX 1.jpg

l to r: pLX007-lacZa^^(PstI/XbaI)(5-25,27-29), Marker

Image:PKU Switch 07-7-15 PLX007-lacZa PX 2.jpg

l to r: Marker, pLX007-lacZa(30,35-53)

那些单切没切开的都是什么?

Transformation of pLX007(sequenced), CD2up-pMD18-T, CD2down-pMD18-T

挑菌SDA-H,Y,cro每板6个

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