Lock & Key By Yu Tao
Acknowledgement and Farewell
- First, I have to say thank you to Mingzhi because he helped me with almost all the experiments these ten days so that I could spend most of my time accompanying my girlfriend. n_n Thank you Mingzhi.
- Second, I am going home today and I am very excited. Goodbye and Work hard, my friends. I like the feeling of home, Ah Ha! :) I will be back on Sep, 1st.
Tandem OriT by Mingzhi Qu & Ze Ren
Conjugation Test:R751_pSC101 X Dh5α_psb1A2(III)
- Donor: C600-R751_psc101 (Tc+)
- Recipient: Dh5α+ psb1A2 (Amp+)
- mixing condition: 220rpm, 90min.
- Get the plates from -4 fridge：C600-R751_psc101(Tc+), C600_psb1A2(Amp+), C600_R751, Dh5α_psc101
- Amplification Culture in liquid LB for 12 hours.
- put 2mL of culture into 20mL of LB with antibiotics, sub-culturing.
Plating the conjugant mix
- C600_R751_psc101 group was set up serial dilutions:original, 10-1, 10-2.
- culture on Tc+/Amp+ plate.
No Donor X recipient time rpm clones(original) clones(e-1) clones(e-2)
1 C600_R751_psc101 X Dh5α_pSB1A2 90 220 full 1000+ <10
2 C600_R751 X Dh5α_pSB1A2 90 220 40+ / /
3 C600_psc101 X Dh5α_pSB1A2 90 220 full / /