IGEM:Paris Liliane Bettencourt/Notebook/Paris 2010/2010/09/09

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Minipreps PmS-MTet-LuxI LuxI attP PsS-RFP PsW-RFP PsM-RFP PmS-RFP pBad/Int Final PmS-MTet x5 LuxI/GFP x1 pLux/LuxI x1 Restriction digest With XbaI and PstI LuxI/GFP I13507/pLux/GFP RBS-GFP-tt (E0240) With SpeI and PstI PmS-MTet-LuxI LuxI pLux/LuxI pLux With EcoRI-HF and PstI PmS-MTet-LuxI PsS-RFP PsW-RFP PsM-RFP PmS-RFP attP Final PmS-MTet x5 Gel electrophoresis PmS-MTet-LuxI (E+P) PsS-RFP PsW-RFP PsM-RFP PmS-RFP attP I13507/pLux/GFP Final PmS-MTet 1 Final PmS-MTet 2 Final PmS-MTet 3 Final PmS-MTet 4 Final PmS-MTet 5 LuxI/GFP RBS-GFP-tt (E0240) Ligations (V + I) 1) Ps + WTet 2) Ps + MTet 3) Ps + STet 4) Ctrl Ps Make competent cells Turbocells (100mL of culture) PmS Chr (to transform with pBad/Int) Transformation of 1-4 + positive control (pUC19) for new Turbocells Overnight cultures Plambda Int From transformations of yesterday : PsS-WTet-LuxI + pBad/Int x2 PmS-MTet-LuxI + pBad/Int x 2