IGEM:Paris Liliane Bettencourt/Notebook/Paris 2010/2010/08/12

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Raphaël and Aleksandra Glycerols, 15% mRBS/TetA(C)+RBS/LuxI, 4 colonies wRBS/TetA(C)+RBS/LuxI, 4 colonies stRBS/TetA(C)+RBS/LuxI, 4 colonies RBS/mRFP/t(K081014)+pLux/GFP, 4 colonies RBS/mRFP/tt(I13507)+pLux/GFP, 4 colonies Ps/wRBS/LuxR +attC/term/attC/mRFP, 3 colonies Ps/mRBS/LuxR +attC/term/attC/mRFP, 4 colonies Pm/sRBS/LuxR +attC/term/attC/mRFP, 1 colony Ps/sRBS/LuxR +attC/term/attC/mRFP, 4 colonies Léa and Théotime Minipreps of 10ml, elution in 35µl warm water mRBS/TetA(C)+RBS/LuxI, 4 colonies wRBS/TetA(C)+RBS/LuxI, 4 colonies stRBS/TetA(C)+RBS/LuxI, 4 colonies RBS/mRFP/t(K081014)+pLux/GFP, 4 colonies RBS/mRFP/tt(I13507)+pLux/GFP, 4 colonies Ps/wRBS/LuxR +attC/term/attC/mRFP, 3 colonies Ps/mRBS/LuxR +attC/term/attC/mRFP, 4 colonies Pm/sRBS/LuxR +attC/term/attC/mRFP, 1 colony Ps/sRBS/LuxR +attC/term/attC/mRFP, 4 colonies Restriction digest mRBS/TetA(C)+RBS/LuxI, 4 colonies with EcoRI-HF and PstI : 5µl miniprep + 2µl B2 + 0.2µl BSA + 12µl water + 2x1µl enzymes wRBS/TetA(C)+RBS/LuxI, 4 colonies with EcoRI-HF and PstI : 5µl miniprep + 2µl B2 + 0.2µl BSA + 12µl water + 2x1µl enzymes stRBS/TetA(C)+RBS/LuxI, 4 colonies with EcoRI-HF and PstI : 5µl miniprep + 2µl B2 + 0.2µl BSA + 12µl water + 2x1µl enzymes RBS/mRFP/t(K081014)+pLux/GFP, 4 colonies with EcoRI-HF and PstI : 5µl miniprep + 2µl B2 + 0.2µl BSA + 12µl water + 2x1µl enzymes RBS/mRFP/tt(I13507)+pLux/GFP, 4 colonies with EcoRI-HF and PstI : 5µl miniprep + 2µl B2 + 0.2µl BSA + 12µl water + 2x1µl enzymes Ps/wRBS/LuxR +attC/term/attC/mRFP, 3 colonies with SpeI and PstI : 5µl miniprep + 2µl B2 + 0.2µl BSA + 12µl water + 2x1µl enzymes Ps/mRBS/LuxR +attC/term/attC/mRFP, 4 colonies with SpeI and PstI : 5µl miniprep + 2µl B2 + 0.2µl BSA + 12µl water + 2x1µl enzymes Pm/sRBS/LuxR +attC/term/attC/mRFP, 1 colony with SpeI and PstI : 5µl miniprep + 2µl B2 + 0.2µl BSA + 12µl water + 2x1µl enzymes Ps/sRBS/LuxR +attC/term/attC/mRFP, 4 colonies with SpeI and PstI : 5µl miniprep + 2µl B2 + 0.2µl BSA + 12µl water + 2x1µl enzymes Gel electrophoresis, 1.5%agarose, for verification only Gel 1 mRBS/TetA(C)+RBS/LuxI col1 mRBS/TetA(C)+RBS/LuxI col2 mRBS/TetA(C)+RBS/LuxI col3 mRBS/TetA(C)+RBS/LuxI col4 wRBS/TetA(C)+RBS/LuxI col1 wRBS/TetA(C)+RBS/LuxI col2 wRBS/TetA(C)+RBS/LuxI col3 wRBS/TetA(C)+RBS/LuxI col4 stRBS/TetA(C)+RBS/LuxI col1 stRBS/TetA(C)+RBS/LuxI col2 stRBS/TetA(C)+RBS/LuxI col3 stRBS/TetA(C)+RBS/LuxI col4 RBS/mRFP/t(K081014)+pLux/GFP col1 RBS/mRFP/t(K081014)+pLux/GFP col2 RBS/mRFP/t(K081014)+pLux/GFP col3 RBS/mRFP/t(K081014)+pLux/GFP col4 File:Gel1208 1.jpg Gel 2 RBS/mRFP/tt(I13507)+pLux/GFP col1 RBS/mRFP/tt(I13507)+pLux/GFP col2 RBS/mRFP/tt(I13507)+pLux/GFP col3 RBS/mRFP/tt(I13507)+pLux/GFP col4 Ps/wRBS/LuxR +attC/term/attC/mRFP col1 Ps/wRBS/LuxR +attC/term/attC/mRFP col2 Ps/wRBS/LuxR +attC/term/attC/mRFP col3 Ps/mRBS/LuxR +attC/term/attC/mRFP col1 Ps/mRBS/LuxR +attC/term/attC/mRFP col2 Ps/mRBS/LuxR +attC/term/attC/mRFP col3 Ps/mRBS/LuxR +attC/term/attC/mRFP col4 Pm/sRBS/LuxR +attC/term/attC/mRFP Ps/sRBS/LuxR +attC/term/attC/mRFP col1 Ps/sRBS/LuxR +attC/term/attC/mRFP col2 Ps/sRBS/LuxR +attC/term/attC/mRFP col3 Ps/sRBS/LuxR +attC/term/attC/mRFP col4