IGEM:Paris Liliane Bettencourt/Notebook/Paris 2010/2010/08/04

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Léa
Miniprep of 10mL cultures elution in 35µL warm water

  • Ps/weak RBS/LuxR
  • Ps/med RBS/LuxR
  • Ps/stand RBS/LuxR
  • Pm/med RBS/LuxR
  • Pm/weak RBS/LuxR
  • Pm/stand RBS/LuxR
  • RBS/LuxI
  • Ps
  • Pm
  • Term


Nanodrop of minipreps

Sample name260/280260/230ng/µL
Ps/weak RBS/LuxR 1.680.76 74.9
Ps/med RBS/LuxR 1.450.55 127.3
Ps/stand RBS/LuxR 1.730.93 62.8
Pm/med RBS/LuxR 1.680.91 134.5
Pm/weak RBS/LuxR 1.470.63 132.1
Pm/stand RBS/LuxR 1.851.60 118.9
RBS/LuxI 1.710.95 134.4
Ps 1.881.37 91.2
Pm 1.791.54 100.4
Term 1.881.65 85.6


Raphaël
Midiprep of 100mL culture

  • attC-t-attC-mRFP


Nanodrop of midiprep

Sample name260/280260/230ng/µL
attC-t-attC-mRFP1.892.27370.2


Aleksandra and Raphaël
Overnight cultures in 10ml

  • attC/term/attC/mRFP #10, 11 cultures - Kan
  • term - Amp
  • RBS/LuxI/term in Turbo cells plate 1, 2 colonies - Amp
  • RBS/LuxI/term in Turbo cells plate 2, 2 colonies - Amp
  • RBS/LuxI/term in TOP10, 3 colonies - Amp


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