IGEM:Paris Liliane Bettencourt/Notebook/Paris 2010/2010/07/07

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Raphaël and Théotime

DNA Gel electrophoresis (agarose 1,0% w/v)
2 µL of EtBr was added in the gel

Protocol (3 wells)

  • 10 µL of minipreped DNA (Qiagen - attC) + 2 µL of loading buffer 6x
  • 10 µL of minipreped DNA (Promega - attC) + 2 µL of loading buffer 6x
  • 10 µL of ladder 1Kb

Migration : 50V, 1h

Image:electrophoresis_070710.jpg
The result is not really conclusive...

Overnight culture
10mL LB

  • pSUlib EX-B0015-attC-S-mRFP1-P (+Kan) 37°C
  • pBad18-IntI1 (+Amp +1% glucose) 37°C
  • pSWK attP-1E (+Cm +dT) 37°C
  • Pi1(+dT) 37°C
  • pTSA CX1 Int lambda (+Amp) 25°C

Plates in the fridge


Antoine

Preparation of plates (Amp + Kan)




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