IGEM:PKU Beijing/2009/Notebook/AND Gate 1/Input/2009/07/07/Hao Wang
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0:34 The digestion reaction begins
7:46 add 0.4uL cip and 2ul buffer to the vector system
8:07 the result come out as follows:
18:03 Recycle the fragments.
19:40 Ligate the 2-4O insert to 1-18P vector:
| Insert | 3μL |
| Vector | 1μL |
| Buffer 10* | 1μL |
| Ligase | 1uL |
| dd H2O | 4μL |
19:48 Ligation start
23:44 Begin the transformation of the ligation plasmid
