IGEM:PKU Beijing/2009/Notebook/AND Gate 1/Input/2009/07/06/Hao Wang

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19:51 Miniprep the plasmids:

2-4OLuxR+RBSAmp+
1-18PP(cat)Kan+

21:08 Digest the plasmids of 2-4O and 1-18P:
Digestion System Insert: 20μL;

Plasmids5μL
Pst11μL
Xbal11ul
Buffer 1*M2μL
dd H2O11μL

Digestion System Vector: 20μL;

Plasmids5μL
Pst11μL
Spe11ul
Buffer 1*H2μL
dd H2O11μL
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