IGEM:PKU Beijing/2009/Notebook/Bistable/2009/07/21/Shan Shen
PCR the clones of high-copy bi-stable system to test if they are correct.
Electrophoresis to test the PCR products.
The order and the amount of the samples: marker 5μL, bi-stable 1, bi-stable 2, bi-stable 3.
All of the three clones are incorrect according to the results of PCR.
Miniprep bi-stable 1,2,3,SupD1 and 1-9H.
|Number of the plasmids||Concentration(ng/μL)|
Digest the bi-stable system to test whether the ligations are correct
Start to digest.
Electrophoresis to test the digestion products.
The order and the amount of the samples: marker 5μL, bi-stable plasmids1, bi-stable 1 digestion products, bi-stable 2 plasmids, bi-stable 2 digestion products, bi-stable plasmids 3, bi-stable 3 digestion products.