IGEM:PKU Beijing/2009/Notebook/Bistable/2009/07/21/Shan Shen
From OpenWetWare
Jump to navigationJump to search
11:00
PCR the clones of high-copy bi-stable system to test if they are correct.
13:30
Electrophoresis to test the PCR products.
The order and the amount of the samples: marker 5μL, bi-stable 1, bi-stable 2, bi-stable 3.
Results:
All of the three clones are incorrect according to the results of PCR.
14:21
Miniprep bi-stable 1,2,3,SupD1 and 1-9H.
Concentrations:
| Number of the plasmids | Concentration(ng/μL) |
| Bi-stable 1 | 160 |
| Bi-stable 2 | 200 |
| Bi-stable 3 | 250 |
| SupD1 | 250 |
| 1-9H | 110 |
16:50
Digest the bi-stable system to test whether the ligations are correct
| Total | 10μL |
| Plasmids | 1μL |
| EcoR1 | 0.5μL |
| Pst1 | 0.5μL |
| Buffer | 1μL |
| ddH2O | 7μL |
17:15
Start to digest.
20:00
Electrophoresis to test the digestion products.
The order and the amount of the samples: marker 5μL, bi-stable plasmids1, bi-stable 1 digestion products, bi-stable 2 plasmids, bi-stable 2 digestion products, bi-stable plasmids 3, bi-stable 3 digestion products.
Results:
