IGEM:PKU Beijing/2009/Notebook/Bistable/2009/07/21/Shan Shen

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11:00
PCR the clones of high-copy bi-stable system to test if they are correct.

13:30
Electrophoresis to test the PCR products.
The order and the amount of the samples: marker 5μL, bi-stable 1, bi-stable 2, bi-stable 3.
Results:

All of the three clones are incorrect according to the results of PCR.

14:21
Miniprep bi-stable 1,2,3,SupD1 and 1-9H.
Concentrations:

Number of the plasmidsConcentration(ng/μL)
Bi-stable 1160
Bi-stable 2200
Bi-stable 3250
SupD1250
1-9H110

16:50
Digest the bi-stable system to test whether the ligations are correct

Total10μL
Plasmids1μL
EcoR10.5μL
Pst10.5μL
Buffer1μL
ddH2O7μL

17:15
Start to digest.

20:00
Electrophoresis to test the digestion products.
The order and the amount of the samples: marker 5μL, bi-stable plasmids1, bi-stable 1 digestion products, bi-stable 2 plasmids, bi-stable 2 digestion products, bi-stable plasmids 3, bi-stable 3 digestion products.
Results:

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