IGEM:PKU Beijing/2009/Notebook/Bistable/2009/07/04/Shan Shen

Prepare the samples of the bi-stable cells:

9:30
Prepare the first sample, the residual was diluted for a fold.
Prepare one sample with the same method each hour.

17:30
Check those samples using the flow cytometer. There aren’t strong red and green controls, so that the results are not convincing.

19:30
Check the parts:

Number	Size of backbone	Size of the insert	Location in the plate	Description	Plasmids
BBa_C0040			1-4A	Tet repressor + LVA
BBa_C0012			1-2O	lacI + LVA
BBa_J09250			2-9B	Constitutive GFP
BBa_C0080			1-14L	araC + LVA
BBa_K093012			3-13M	Constitutive GFP
BBa-J3033			2-4O	B0034 + LuxR
BBa_R0010			1-1D	LacI regulated promoter
BBa_B0025			1-2E	Double terminators
BBa_I14033			1-18P

22:30
Transformation for the parts above.

1:45
The incubation was started.

2:00
Pick the pZSA GREEN and RED for incubation, 3-13M as a red control, 2-9B as a green control, 1-1D as a negative control.