IGEM:PKU Beijing/2009/Notebook/AND Gate 1/Output/2009/06/23/Zhangs

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Double digestion for Terminator vector
Digestion system:

30μLTerminator plasmid
5μL10×M beffer
1μLEcoRI
1μLXbaI
13μLddH2O
50μLTotal

After 90min digestion, add: 1~2μL CIAP+5μL CIAP buffer

Ligate the CI insert and Terminator vector.

Transforming the CI insert + Terminator vector ligation product.

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