IGEM:PKU Beijing/2009/Notebook/AND Gate 1/Output/2009/06/22/Zhangs
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Double digestion for CI insert
Digestion system:
| 30μL | CI plasmid |
| 5μL | 10×H beffer |
| 1μL | EcoRI |
| 1μL | SpeI |
| 13μL | ddH2O |
| 50μL | Total |
Electrophoresis:
50μL system + 10μL buffer
5μL marker + 1μL buffer
Sample loading order: DL2000 marker, CI1, CI2
Extraction the nucleic acids from gel.
