IGEM:PKU Beijing/2009/Notebook/AND Gate 1/Input/2009/08/02/Rencheng Gao

11:30
Pouring plates: LB+Amp ×30, LB+Kan ×50

15:30
Mini-prep: pla1~5, pcr1~4
Gel electrophoresis

17:00
Double-enzyme digestion (front insert)
Plasmid: pla1, pla2, pcr1, pcr3

22:30
Gel purification (pcr1, pla1, pla2)

00:00
Ligation:
Insert: pcr1, pla1
Vector: SupD, 1-12O