IGEM:PKU Beijing/2009/Notebook/AND Gate 2/2009/07/23/Zhenglin Yang
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10:40 PCR production purify
12:30 Double digestion for purified T3 Promoter PCR Production
16:30 Purify the digestion production
17:30 Ligation of T3 promoter and GFP vector
18:00 Miniprep for T3 Polymerase
21:30 Ligation of T3 promoter and TetR backbone (T3 promoter parts)
22:00 Transformation of T3 promoter + GFP
23:00 PCR for T7 polymerase
23:30 Plate cultivation of T3 promoter + GFP
