IGEM:PKU Beijing/2009/Notebook/AND Gate 1/Input/2009/07/16/Min Lin

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MiniPrep 1-18P and 2-4O plasmid
Enzyme Digestion of 1-18P and 2-4O plasmid

SpeI1ul
PstI1ul
10xH buffer2ul
1-18P Plasmid2ul
ddH2O13ul
XbaI1ul
PstI1ul
10xM buffer2ul
2-4O Plasmid10ul
ddH2O6ul


Several Trials and failures, 2-4O can not be digested normally, at least be TaKaRa Enzymes.
Check up the parts and found
BBa_J09855 Constitutive LuxR with pLuxR 1-9H pSB1A2

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