IGEM:PKU Beijing/2009/Notebook/AND Gate 1/Input/2009/07/09/Hao Wang

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9:06 Miniprep the 2-4O->1-18P plasmids

11:52 Digest the plasmids of 2-4O->1-18P and 1-23L:
Digestion System Insert: 20μL;

Plasmids5μL
EcoR11μL
Spe11ul
Buffer 1*H2μL
dd H2O11μL

Digestion System Vector: 20μL;

Plasmids5μL
EcoR11μL
Xbal11ul
Buffer 1*M2μL
dd H2O11μL

12:13 Reaction Start

17:32 Stop the digestion

20:08 Prepare to Ligation

20:17 Ligation start

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