IGEM:PKU Beijing/2009/Notebook/AND Gate 1/Input/2009/07/08/Min Lin
The Primer(Made of Standard Prefix and Suffix arrived).
Add ddH2O to each tube.
Try Colony PCR
Go to Lingli’s Lab, do gradient PCR.
52, 53, 54, 55, 56, 57, 62, 64
It turns out that the thermocycler in our lab has no hot cap. So the liquid is on the tube cap when exposed to 94 centigrade.