IGEM:Missouri University of Science and Technology/2009/Notebook/Cyanobacteria Mediated Filtration of Coal Flue/2014/09/27

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Date: 9/27/14 People in lab: Kelsey Crossen, Kira Buckowing

Title: Transformation of hmp into competent cells Start Time: 10:10 am Purpose: Transform ligation products into comp cells Protocol: LTM Ed. 2 Chemical Transformations Exceptions: Products:

Sample Label Description Source Label Quantity
CT1 9/27 20 uL L1 9/26 1
CT2 9/27 200 uL L1 9/26 1
CT3 9/27 20 uL L2 9/26 1
CT4 9/27 200 uL L2 9/26 1
CT5 9/27 20 uL L3 9/26 1
CT6 9/27 200 uL L3 9/26 1
CT7 9/27 20 uL L4 9/26 1
CT8 9/27 200 uL L4 9/26 1
CT9 9/27 20 uL MP2 8/9 1
CT10 9/27 200 uL MP2 8/9 1

Results: (9/28) Growth on all plates! Notes: Tubes were incubated for a bit over 2 hours (~10-15 min) Stop Time: 2 pm Next: Start liquid culutures of any colonies that grow




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