IGEM:Missouri University of Science and Technology/2009/Notebook/Cyanobacteria Mediated Filtration of Coal Flue/2014/09/16

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Date: 9/16/14 People in lab: Kelsey Crossen

Title: Ligation of hmp and pro+RBS backbone Start Time: 2:05 pm Purpose: To ligate hmp into vector for transformation into comp cells Protocol: LTM Ed. 2 Ligation Exceptions: L1 - 2 uL vector, 6 uL insert; L2 = 4 uL vector and insert; L3 = 2 uL insert and 4 uL vector Products:

Sample Label Description Source Label Quantity
9/16 L1 hmp and pro+RBS 8/11 GE1 and 8/13 GE1 1
9/16 L2 hmp and pro+RBS 8/11 GE1 and 8/13 GE1 1
9/16 L3 hmp and pro+RBS 8/11 GE1 and 8/13 GE1 1




Results: Notes: Stop Time: 2:35 pm Next: Chemical transformations



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