IGEM:Missouri University of Science and Technology/2009/Notebook/Cyanobacteria Mediated Filtration of Coal Flue/2014/08/27

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Date: 8/27/14 People in lab: Kelsey Crossen

Title: Transformation of hmp and pro+RBS backbone and RFP in chlor resistant backbone to test comp cells Start Time: 2 pm Purpose: To test if comp cells are working by running a control with the target reaction for the project Protocol: LTM Ed. 2 Chemical Transformation Exceptions: 1.5 uL RFP and 1.5 uL Ligation product used and run in the same transformation Products:

Sample Label Description Source Label Quantity
CT1 8/27 20 uL L1 8/18 and DH5 alpha 2013 DJW 1
CT2 8/27 200 uL L1 8/18 and DH5 alpha 2013 DJW 1
CT3 8/27 20 uL RFP in chlor bb stock 12/18/13 1
CT4 8/27 200 uL RFP in chlor bb stock 12/18/13 1


Results: (8/29) No growth - comp cells are deemed bad Notes: DH5 alpha cells used from bottom shelf of -80 C freezer Stop Time: 5 pm Next: Comp cells need to be remade



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