IGEM:Missouri University of Science and Technology/2009/Notebook/Cyanobacteria Mediated Filtration of Coal Flue/2014/07/06

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Date: 7/6/14 People in lab: Emily Puleo, Joshua Heath

Title: PCR of hmp Start Time: 10 am Purpose: Amplify PCR with the iGEM prefix and suffix Protocol: LTM Ed. 2 PCR Exceptions: 7.5 uL template DNA, 2.5 uL primers Products:

Sample Label Description Source Label Quantity
hmp PCR 7/6 A hmp with prefix and suffix --- 1
hmp PCR 7/6 B hmp with prefix and suffix --- 1




Results: Notes: Stop Time: 1:45 pm Next: Gel electrophoresis to check products

Date: 7/6/14 People in lab: Emily Puleo, Joshua Heath

Title: Gel electrophoresis of hmp PCR 7/6 A and B Start Time: 2:45 pm Purpose: Check PCR product Protocol: LTM Ed. 2 Gels Exceptions: Products:

Well 1 2 3 4 5 6 7 8
- - 7/6 hmp A - Ladder - 7/6 hmp B -

Products: Results: Notes: Stop Time: 3:30 pm Next: TOPO

Date: 7/6/14 People in lab: Emily Puleo, Joshua Heath

Title: TOPO cloning of hmp products Start Time: 1:55 pm Purpose: To make more copies of the PCR product Protocol: Invitrogen TOPO cloning kit manual Exceptions: Products:

Sample Label Description Source Label Quantity
A1 --- hmp PCR 7/6 A 1
B1 --- hmp PCR 7/6 B 1




Results: Notes: Stop Time: 2:10 pm Next: Transformation

Date: 7/6/14 People in lab: Emily Puleo, Joshua Heath

Title: Chemical Transformation of hmp Start Time: 2:10 pm Purpose: Blue/white screening Protocol: LTM Ed 2 Chemical Transformation Exceptions: Let xgal diffuse through agar for 1 hour Products:

Sample Label Description Source Label Quantity
7/6 CT A-1 20 uL A1 1
7/6 CT A-2 200 uL A1 1
7/6 CT B-1 20 uL B1 1
7/6 CT B-2 200 uL B1 1


Results: Notes: Commercial competent cells used Stop Time: 4:40 pm Next: Blue/white screening



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