IGEM:Missouri University of Science and Technology/2009/Notebook/Cyanobacteria Mediated Filtration of Coal Flue/2014/06/07

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Date: 6/7/14 People in lab: Emily Puleo, Kira Buckowing, Caleb Treccazi

Title: PCR of hmp gene Start Time: 10:30 am Purpose: To add the iGEM prefix and suffix Protocol: LTM Ed. 2 PCR Exceptions: Products:

Sample Label Description Source Label Quantity
6/7 hmp A --- --- 1
6/7 hmp B --- --- 1




Results: Notes: Stop Time: 11 am Next: Gel electrophoresis

Date: 6/7/14 People in lab: Emily Puleo, Kira Buckowing, Caleb Treccazi

Title: Gel electrophoresis of hmp PCR products Start Time: 11:30 am Purpose: To check for products Protocol: LTM Ed. 2 Gel Exceptions: Products:

Well 1 2 3 4 5 6 7 8
- - 6/7 hmp A - Ladder - 6/7 hmp B -

Products: Results: Notes: Stop Time: 12: 15 pm Next: TOPO

Date: 6/7/14 People in lab: Emily Puleo, Kira Buckowing, Caleb Treccazi

Title: TOPO cloning of hmp Start Time: 11:45 am Purpose: To make more copies of amplified hmp Protocol: LTM Ed. 2 Chemical Transformations and Invitrogen TOPO cloning kit manual Exceptions: Products:

Sample Label Description Source Label Quantity
6/7 CT hmp A1-1 20 uL 6/7 hmp A 1
6/7 CT hmp A1-2 200 uL 6/7 hmp A 1
6/7 CT hmp A2-1 20 uL 6/7 hmp A 1
6/7 CT hmp A2-2 200 uL 6/7 hmp A 1
CT hmp B1-1 20 uL 6/7 hmp B 1
CT hmp B1-2 200 uL 6/7 hmp B 1
CT hmp B2-1 20 uL 6/7 hmp B 1
CT hmp B2-2 200 uL 6/7 hmp B 1

Results: (6/8) No growth seen - cells not competent or died? Notes: Stop Time: 3:30 pm Next: Make competent cells (ran out); try PCR once more



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