IGEM:Missouri University of Science and Technology/2009/Notebook/Cyanobacteria Mediated Filtration of Coal Flue/2014/03/19

Cyanobacteria Mediated Filtration of Coal Flue Gas

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Date: 03/19/14 People in lab: Levi Palmer, Caleb Trecazzi Title: PCR of hmp and TOPO-TA cloning with transformation Start Time: 4:00 PM Purpose: To amplify hmp gene from E. coli and put into TOPO vector to transform into E. coli(DH5alpha) Protocol: LTM ed. 2, Invitrogen TOPO-TA cloning manual Exceptions: 1. 6min incubation time Products: Sample Label Description Source Label Quantity hmp TOPO 3/19 1 hmp in TOPO vector, plated 1uL PCR product used E.coli 8/6/13 4 hmp TOPO 3/19 2 hmp in TOPO vector, plated 2.5uL PCR product used E.coli 8/6/13 4 hmp TOPO 3/19 3 hmp in TOPO vector, plated 4uL PCR product used E.coli 8/6/13 4 Results: Notes: Variable amounts of PCR product used to see what works best. Stop Time: 7:00 PM Next: Blue/white colony screening to check for colonies with insert