IGEM:Missouri University of Science and Technology/2009/Notebook/Cyanobacteria Mediated Filtration of Coal Flue/2014/03/18

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Date: 03/18/14 People in lab: Levi Palmer, Caleb Trecazzi

Title: PCR of 16S ribosomal subunit from Genome prep, gel electrophoresis of products

Start Time: 2:00 PM

Purpose: To confirm usable DNA from the genome prep

Protocol: LTM ed. 2 Exceptions: 1. 2uL of each primer used, 2. No DNA and no primer controls. 3. Annealing temp: 50C 4. Elongation time: 3min

Well 1 2 3 4 5 6 7 8
Sample Sans primer S16 S16 Sans DNA

Results: S16 bands present, controls absent.

Stop Time: 7:00 PM

Next: Retry norCB PCR with different temp



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