IGEM:Missouri University of Science and Technology/2009/Notebook/Cyanobacteria Mediated Filtration of Coal Flue/2013/09/14
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Date: 09/14/13 People in lab: Hannah FryeTitle: PCR of norCB an nosZ for TOPO TA cloning Start Time: Purpose: Add iGEM prefix&suffix to the genes, then TOPO-TA clone to amplify Protocol: 1. Resuspended P. aeru cells in0.5mL milliQ 2. Spun down Taq Master Mix 3. Mixed - 12.5 uL Taq MM, 7.5 uL P. Aeru, 2.5 uL F primer, 2.5 uL R primer 4. Spun down mixture 5. Annealing Temp: 65C, Elongation Time: 7 min Products:
Stop Time: Next: Gel electrophoresis
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