Description
- Our goal is to engineer a common yogurt bacteria, Lactobacillus bulgaricus, so that it will express the 20aa peptide p1025. A clinical study (Kelly CG et al.; Nature Biotechnol. 1999) reports that p1025 is good for your teeth. p1025 reduces oral colonization of Streptococcus mutans, a tooth-decaying bacterium.
- Stage 1: Construction of p1025 fusion peptide and expression of gene in E. coli. This is an intermediate step to evaluate gene function and protein secretion/efficacy.
- Stage 2: Binding Assay - see if the p1025 produced by E.coli inhibits binding of S. mutants to hydroxyapatite (HA) beads.
- Stage 3: Expression of p1025 in Lactobacillus.
- Stage 4: Make yogurt with modified Lactobacillus and test for inhibition of S. mutants binding.
Notes
- Group meetings every Friday at 4pm in 68-474 (except 7/11 and 8/1 in 68-674)
- Journal club meetings as needed every Friday at 11am in 68-474
Information
Useful Links
- |DNA Plasmid Editor
- |Peptide Sequence Manipulations
- Primer Analyzer Tool (check temperature & GC content) | One | Two
- PMID 9920267
A synthetic peptide adhesion epitope as a novel antimicrobial agent.
- PMID 9062560
Inhibition of Streptococcus mutans adsorption to hydroxyapatite by low-molecular-weight chitosans.
- PMID 12788739
(a secretion signal sequence described; can use the promoter, RBS and secretion signal for this protein for p1025).
- PMID 11772607
Electrotransformation of Lactobacillus delbrueckii subsp. bulgaricus and L. delbrueckii subsp. lactis with various plasmids.
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