IGEM:MIT/2007/Notebook/2007-7-27

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Contents

Agenda

  • dilute LC's of Mer/GFP Transformations and BL21 untransformed cells
    • Miniprep LC's of Mer/GFP
  • Finish Western Blot
  • Order EC20 oligos

EC_20 Oligos

  • Ordered with IE-HCLG purification, should arrive Tuesday

Polystyrene Assay

Wells:
(Done in Triplicate)
CPX(-5 AHL) CPX(-6 AHL) CPX(-7 AHL) CPX(-8 AHL) BLANK WELL PICCS8(1/500) Neg Control(Uninduced)
Diagram of the Polystyrene Assay Protocol
Diagram of the Polystyrene Assay Protocol

E-5a

E-5b

E-5c

E-6a

E-6b

E-6c

E-7a

E-7b

E-7c

E-7d

E-7e

E-7f

E-8a

E-8b

E-8c

E-8d

E-8e

E-8f

Control 1

Control 2

Control 3

Image 21

Diagram of the Polystyrene Assay Protocol
Diagram of the Polystyrene Assay Protocol

Western Blot Results

A pdf of the blots

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