IGEM:MIT/2007/Notebook/2007-6-15

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Contents

Grad Advisors

  • Morning: Debbie, Forrest
  • Afternoon: Brian

LAB WORK

Previous night:

  • Analyze plates
  • innoculate 4ml LB/Amp

Day of:

  • Mini-prep plasmid DNA

Results from last night's incubation

Contents    Volume    Label    # of Colonies    
U V         (2 µL)    I        67
ddV         (5 µL)    A        0
ddV + ddI   (5 µL)    B        0
dV          (5 µL)    C        28               
dV + dI     (5 µL)    D        49
ddV         (10 µL)   E        0
ddV + ddI   (10 µL)   F        5
dV          (10 µL)   G        0
dV + dI     (10 µL)   H        5
  • All plates with colonies have feeder colonies

Plan

  1. Plate and look at them.
  2. At least a couple colonies.
  3. if lots, pick 2 colonies from each; doesn't matter 5 ul or 10 ul. Just need 4 different types
  4. One colony into one tube, and repeat. So we have 11 innoculation tubes; 2 for each type and all 5 for ddV/ddI.
  5. Put LB amp into glass tubes with metal caps (flamed)
  6. Loop and insert colonies.
  7. Place plates in fridge
  8. Place tubes in 37°C room overnight
  9. Store in refigerator for mini-prep on monday

Tubes:

  • 2: U V
  • 2: dV
  • 2: dV + dI
  • 5: ddV + ddI
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