IGEM:MIT/2006/Notebook/2007-7-19

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To Do Today

1. Add PCNB to all GC samples- DONE

2. Run all EIGHTEEN GC samples- DONE

3. Take out plate of IlvE- strain- DONE

4. Take out new LCs of J45120 and J45181- DONE

5. Adjust the pH of Tom's media as Tom suggested- DONE (looks to be ~7.7)

6. Grow up the same cultures on Tom's pH-adjusted media- DONE

7. Add 50 uL water to tube containing Japanese plasmid- DONE

8. Nanodrop the contents of the tube containing Japanese plasmid- DONE (21.6 ng/uL DNA)

Results

J45120

Hour-----------OD600------------Methyl Salicylate (ppm)-------Methyl Salicylate (ppm)/OD600

5--------------0.14-------------0.0---------------------------0.00

6--------------0.42-------------0.0---------------------------0.00

7--------------0.84-------------0.0---------------------------0.00

8--------------1.38-------------1.0---------------------------0.72

9--------------1.84-------------23.1--------------------------12.6

10------------2.20--------------28.7--------------------------13.1

11------------2.26--------------26.1--------------------------11.6

12------------2.40--------------27.5--------------------------11.5

13------------2.48--------------18.8--------------------------7.58


J45181

Hour----------OD600-------------Methyl Salicylate (ppm)-------Methyl Salicylate (ppm)/OD600

5--------------0.24-------------0.0---------------------------0.00

6--------------0.50-------------1.1---------------------------2.20

7--------------0.92-------------8.7---------------------------9.46

8--------------1.46-------------18.5--------------------------12.7

9--------------1.82-------------22.3--------------------------12.3

10------------2.30--------------25.9--------------------------11.3

11------------2.20--------------24.8--------------------------11.3

12------------2.38--------------24.8--------------------------10.4

13------------2.52--------------18.3--------------------------7.26

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