IGEM:MIT/2006/Notebook/2006-8-21

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Ligations

Ligate osmYES with XP cut:

  1. Q99ABC
  2. Q119ABC
  3. Q199ABC
  4. Q219ABC
  5. QE0840ABC

(after running a gel to make sure inverter Q044000 is around)

Mutagenesis

Did mutagenesis on 30-BAT2-30-THI3 to get rid of site in THI3 and on pchBA-15

Transformations

Transform ligations and mutagenesis products

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