IGEM:MIT/2006/Notebook/2006-7-27

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Contents

To Do

  1. Miniprep overnight cultures
  2. Ligations + Digestions
  3. Pour more LB Amp plates
  4. Retransform pchBA and osmY promoter + pSB1A3-1
  5. Transform ligations

Digestions

  1. E0840 w/ EX
  2. osmY w/ S
  3. ATF1-1148 w/ ES
  4. B0030 w/ SP
  5. B0032 w/ SP

Ligations

  1. B0030(2)SP + 45001.B0015CXP
  2. B0030(2)SP + ATF1-1148XP
  3. osmYES + E0840EX
  4. ATF1-1148ES + B0015EX

Transformations (using new cells from Tom)

  1. B0030(2)SP + 45001.B0015CXP
  2. B0030(2)SP + ATF1-1148XP
  3. osmYES + E0840EX
  4. ATF1-1148ES + B0015EX
  5. pchBA w/ pchA silent mutation (transformation failed yesterday)
  6. osmY + pSB1A3 backbone (transformation failed yesterday)
  7. pUC19

Desired Completed Constructs

  1. RBS-SAMT-Term
  2. RBS-ATF1
  3. SPP-RBS-GFP-Term
  4. ATF1-Term

Listed Current Biobricks

Can be found at in the index

Made liquid cultures of transformants

  • R0040+B0030 [A, B, C, and D] (in LBAmp)
  • R0040+B0032 (in LBAmp)
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