IGEM:MIT/2006/Autotrophic E.coli/Biobrick Calvin cycle enzymes
- prkA contains a Pst1 site
- rbcL contains an EcoR1 site
- Miniprep AE4 (this will provide a plasmid prep of both enzymes).
- Site-directed mutagenesis of the prkA and rbsLS
- Sequence verify that we got mutations
- Cut out the coding regions with primers containing BB ends.
- Clone into BB vector.