IGEM:MIT/2005/Ligand (substance that is being sensed)

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Goals

  • Understand what piece of DNA should be used between the fluorescein molecules
  • Learn specifics of primer design
  • Decide which company to order from (e.g. idtdna) and figure out specifics of ordering
  • Determine if RE sites should be added into the piece of DNA (for negative testing of dimerization)
  • Figure out how the ligand can enter the outer membrane of the e.coli cell
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