IGEM:MIT/2005/Colby Discussion
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- What antigen do we want to attack? Anything.
- Tricky part: having generic chain to signal the event.
- To detect surface binding
- Flow cytometry to detect presence of antigen
- George Georgiou at UT.
- Hellinga at Duke.
- Induction systems - gene. mod. cell that binds to pheromone to gene expression.
- Modifications made to fuse antibody: no modifications at all. Just fuse DNA to make fusion protein.
- Usu. use single chain antibody, not entire igg. Just through binding.
- "Antibody Structure" to see more ideas about domains.
- Mechanisms:
- voltage-gated channels
- ligang-gated channels
- dimerization
- 2 antibodies fuse to 2 diff. cell units and having antibody rec different parts to bring subunits close together to bind.
- feasibility: depends on size of antigen; protein = easy; 15-20 aa. Small molecule would be harder (probably not).
- Generic system = potentially difficult. Only limits of proteins.
- 2 antibodies fuse to 2 diff. cell units and having antibody rec different parts to bring subunits close together to bind.
- cleavage of transcription factor
- g proteins